PHOX2B Monoclonal antibody

PHOX2B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1F5F5

Cat no : 66254-1-Ig

Synonyms

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B

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Validation Data Gallery

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  WB analysis of Neuro-2a using 66254-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66254-1-Ig (PHOX2B Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of neuroblastoma using 66254-1-Ig

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 40x lens).

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  IHC staining of neuroblastoma using 66254-1-Ig

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 10x lens).

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  IF Staining of Neuro-2a using 66254-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of SH-SY5Y using 66254-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: Neuro-2a cells
• Positive IHC detected in: neuroblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells, Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

66254-1-Ig targets PHOX2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PHOX2B fusion protein Ag13196
• Full Name: paired-like homeobox 2b
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC017199
• Gene Symbol: PHOX2B
• Gene ID (NCBI): 8929
• RRID: AB_2881642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

Publications

Species	Application	Title
human	IF	Sci Signal; Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion.; Authors - Ouranio Anastasiou
mouse	IF	Nat Biotechnol; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells; Authors - Yunlong Tao