HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15109-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15109-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 antibody) at dilution of 1:300.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 antibody) at dilution of 1:300.
IHC staining of human liver cancer using 15109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 15109-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 15109-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15109-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 15109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 15109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using 15109-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 antibody) at dilution of 1:300.
IF/ICC Figures
IF Staining of HEK-293 using 15109-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 15109-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PHKG2 Polyclonal antibody
Catalog Number
15109-1-AP
Citations
1
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
