PHIP Polyclonal antibody, PBS Only

PHIP Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20933-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 20933-1-AP (same clone as 20933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 20933-1-AP (same clone as 20933-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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  IP experiment of A375 using 20933-1-AP (same clone as 20933-1-PBS)

  IP result of anti-PHIP (IP:20933-1-AP, 4ug; Detection:20933-1-AP 1:3000) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 20933-1-AP (same clone as 20933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 20933-1-AP (PHIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20933-1-PBS in a different storage buffer formulation.

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Product Information

20933-1-PBS targets PHIP in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHIP fusion protein Ag14968
• Full Name: pleckstrin homology domain interacting protein
• Calculated Molecular Weight: 1821 aa, 207 kDa
• Observed Molecular Weight: 207 kDa
• GenBank Accession Number: BC008909
• Gene Symbol: PHIP
• Gene ID (NCBI): 55023
• RRID: AB_10733522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.