PHF8 Polyclonal antibody, PBS Only

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  WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 29516-1-AP (PHF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

PHF8 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29516-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

29516-1-PBS targets PHF8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF8 fusion protein Ag30701
• Full Name: PHD finger protein 8
• Calculated Molecular Weight: 118 kDa
• Observed Molecular Weight: 125-135 kDa
• GenBank Accession Number: NM_001184896
• Gene Symbol: PHF8
• Gene ID (NCBI): 23133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UPP1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Plant homeodomain finger protein 8 (PHF8) is a JmjC domain-containing protein. PHF8 is localized in the nucleolus and may regulate rRNA transcription. As a member of the KDM7 subfamily of enzymes, PHF8 is involved in demethylation of lysine residues in histones such as H3K27me2/1, H3K9me2/1 and H4K20me1. PHF8 has been reported to participate in cancer development and metastasis of various types of tumors(PMID: 33111613). PHF8 has 5 isoforms produced by alternative splicing(Uniprot).