PHF20 Polyclonal antibody, PBS Only

PHF20 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22010-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 22010-1-AP (same clone as 22010-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 22010-1-AP (same clone as 22010-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 22010-1-AP (same clone as 22010-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22010-1-AP (PHF20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

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Product Information

22010-1-PBS targets PHF20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF20 fusion protein Ag15686
• Full Name: PHD finger protein 20
• Calculated Molecular Weight: 1012 aa, 115 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC150178
• Gene Symbol: PHF20
• Gene ID (NCBI): 51230
• RRID: AB_2878970
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PHD finger protein 20 (PHF20),also known as NZF, TZP, GLEA2 or HCA58, is a 1,012 amino acid protein that localizes to the nucleus and contains one PHD-type zinc finger, one C2H2-type zinc finger and one A/T hook DNA-binding domain.PHF20 contains a tudor domain, which facilitates binding to di-methylated histone H4 Lys20. PHF20 is expressed in a variety of tissues, including liver, heart, lung, pancreas, spleen, testis and placenta, PHF20 is thought to function as a transcription factor, possibly playing a role in the development of cancers, including glioblastoma, lung cancer, colon cancer and ovarian cancer. PHF20 exists as three alternatively spliced isoforms (150kd,130kd and 120kd) which are encoded by a gene that maps to human chromosome 20 (22072714).