PHF14 Polyclonal antibody

• Featured Product
• KD/KO Validated

PHF14 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 24787-1-AP

Synonyms

KIAA0783, PHD finger protein 14, PHF14

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Validation Data Gallery

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  WB analysis of HeLa using 24787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24787-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 24787-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24787-1-AP (PHF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 24787-1-AP

  IP result of anti-PHF14 (IP:24787-1-AP, 4ug; Detection:24787-1-AP 1:500) with HEK-293 cells lysate 1200ug.

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  IHC staining of human kidney using 24787-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24787-1-AP (PHF14 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 24787-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24787-1-AP (PHF14 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MDA-MB-453s cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Product Information

The immunogen of 24787-1-AP is PHF14 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF14 fusion protein Ag20542
• Full Name: PHD finger protein 14
• Calculated Molecular Weight: 888 aa, 100 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC152414
• Gene Symbol: PHF14
• Gene ID (NCBI): 9678
• RRID: AB_2879724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHF14 also named as KIAA0783 or PHD finger protein 14 is 888 amino acid protein, which contains 2 PHD-type znic fingers. PHF14 localizes in nucleus and may be involved in the regulation of transcription, DNA dependent. The function of PHF14 is still non-known.

Publications

Species	Application	Title
human,mouse	WB,CoIP,ChIP	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	WB	Elife; Integrated analysis of H2A.Z isoforms function reveals a complex interplay in gene regulation.; Authors - Assala Lamaa
human	WB,IHC	Biomed Res Int; PHF14 Promotes Cell Proliferation and Migration through the AKT and ERK1/2 Pathways in Gastric Cancer Cells.; Authors - Yuzu Zhao ; KD Validated
human	WB,CoIP,ChIP	Nucleic Acids Res; The high mobility group protein HMG20A cooperates with the histone reader PHF14 to modulate TGFβ and Hippo pathways; Authors - Elena Gómez-Marín ; KD Validated
human	WB,IHC,IF,ChIP	Cell Discov; PHF14 enhances DNA methylation of SMAD7 gene to promote TGF-β-driven lung adenocarcinoma metastasis; Authors - Han Tian
human	WB	Mol Cancer Res; SP4 facilitates esophageal squamous cell carcinoma progression by activating PHF14 transcription and Wnt/β-Catenin signaling; Authors - Li Wei ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jose C. (Verified Customer) (10-14-2019)

The antibody gives good results in WB, IP and co-IP. We have verified by siRNA that the identified band correspond to PHF14.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: MDA-MB-231, HEK293T and MCF7

PHF14 Antibody Western Blot,Immunoprecipitation, validation (1/500 dilution) in MDA-MB-231, HEK293T and MCF7 (Cat no:24787-1-AP)