PHF11 Polyclonal antibody

• Featured Product
• KD/KO Validated

PHF11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10898-1-AP

Synonyms

APY, IGEL, IGER, IGHER, NY REN 34, NYREN34, PHD finger protein 11, PHF11

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Validation Data Gallery

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  WB analysis of HeLa using 10898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10898-1-AP (PHF11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10898-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10898-1-AP (PHF11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10898-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10898-1-AP (PHF11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 10898-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10898-1-AP (PHF11 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse thymus tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10898-1-AP targets PHF11 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF11 fusion protein Ag1318
• Full Name: PHD finger protein 11
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC017212
• Gene Symbol: PHF11
• Gene ID (NCBI): 51131
• RRID: AB_2165207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHF11 is a transcriptional co-activator of the Th1 effector cytokine genes, interleukin-2 (IL2) and interferon-γ (IFNG), co-operating with nuclear factor kappa B (NF-κB). The reduction in PHF11 expression seen with an AD-associated genotype could contribute to the strong T(H)2 responses that characterize many allergic individuals.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.; Authors - Mehdi Eshraghi
human	WB,IP	J Allergy Clin Immunol; Functional characterization of the atopy-associated gene PHF11.; Authors - Clarke Emily E
human	WB	PLoS Pathog; Inhibition of spumavirus gene expression by PHF11.; Authors - Melissa Kane
mouse	WB	iScience; Analysis of microbiota reveals the underlying mechanism of PHF11 in the development of Enterococcus-regulated endometriotic cysts; Authors - Jingjing Hu
human	WB	Immunol Cell Biol; A role for the atopy-associated gene PHF11 in T-cell activation and viability.; Authors - Rahman Nusrat N ; KD Validated
human	IF	BMC Immunol; PHF11 expression and cellular distribution is regulated by the Toll-Like Receptor 3 Ligand Polyinosinic:Polycytidylic Acid in HaCaT keratinocytes.; Authors - Pauline Muscat ; KD Validated