PHEX Monoclonal antibody, PBS Only

PHEX Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4B2

Cat no : 66934-1-PBS

Synonyms

HPDR, HPDR1, HYP, HYP1, PHEX, XLH

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 66934-1-Ig (same clone as 66934-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat lung using 66934-1-Ig (same clone as 66934-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig lung using 66934-1-Ig (same clone as 66934-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66934-1-Ig (same clone as 66934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66934-1-Ig (PHEX antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

---

Product Information

66934-1-PBS targets PHEX in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Pig samples.

• Tested Reactivity: Human, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PHEX fusion protein Ag20290
• Full Name: phosphate regulating endopeptidase homolog, X-linked
• Calculated Molecular Weight: 749 aa, 86 kDa
• Observed Molecular Weight: 86-100 kDa
• GenBank Accession Number: BC105059
• Gene Symbol: PHEX
• Gene ID (NCBI): 5251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660).Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).