Prohibitin Polyclonal antibody

• Featured Product
• KD/KO Validated

Prohibitin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (2)

Cat no : 10787-1-AP

Synonyms

PHB, Prohibitin 1, PHB1

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Validation Data Gallery

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  WB analysis using 10787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10787-1-AP (Prohibitin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10787-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10787-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10787-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10787-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10787-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10787-1-AP (PHB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10787-1-AP (Prohibitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10787-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10787-1-AP (PHB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10787-1-AP (Prohibitin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 10787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Prohibitin antibody (10787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, human liver tissue, human brain tissue, NIH/3T3 cells, HeLa cells, Raji cells, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat kidney tissue
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: H9C2 cells, HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

10787-1-AP targets Prohibitin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Prohibitin fusion protein Ag1240
• Full Name: prohibitin
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC013401
• Gene Symbol: Prohibitin
• Gene ID (NCBI): 5245
• RRID: AB_2164476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

Publications

Species	Application	Title
human	WB	Cell; Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts.; Authors - Bei-Bei Chu
human	WB	Autophagy; PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.; Authors - Chaojun Yan
mouse	WB	Cell Death Differ; Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.; Authors - H Li
human	WB	Hum Mol Genet; Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.; Authors - McCray Brett A BA
mouse	WB	Cell Mol Gastroenterol Hepatol; DJ-1 deficiency in hepatocytes improves liver ischemia-reperfusion injury by enhancing mitophagy.; Authors - Min Xu
human	WB	Elife; Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.; Authors - Sven Dennerlein

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (10-10-2024)

It works well in immunofluorescence using mouse primary lymphocytes. It localises to the mitochondria.

• Applications: Immunofluorescence

Prohibitin Antibody Immunofluorescence validation ( dilution) in (Cat no:10787-1-AP)

FH

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate. Membrane was blocked in 5% BSA for 1 hour then incubated overnight at 4 degrees with the primary antibody (1:1000)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

Prohibitin Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10787-1-AP)