PGRMC2 Polyclonal antibody

PGRMC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24575-1-AP

Synonyms

PMBP, DG6

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Validation Data Gallery

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  WB analysis using 24575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24575-1-AP (PGRMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24575-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24575-1-AP (PGRMC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 24575-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24575-1-AP (PGRMC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 24575-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24575-1-AP (PGRMC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24575-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGRMC2 antibody (24575-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, SKOV-3 cells, human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24575-1-AP targets PGRMC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGRMC2 fusion protein Ag20077
• Full Name: progesterone receptor membrane component 2
• Calculated Molecular Weight: 223 aa, 24 kDa
• Observed Molecular Weight: 22 kDa, 28 kDa
• GenBank Accession Number: BC016692
• Gene Symbol: PGRMC2
• Gene ID (NCBI): 10424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 is highly homologous to PGRMC1. PGRMC2 might play a role in cancer.