PGP Polyclonal antibody

PGP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (2)

Cat no : 25081-1-AP

Synonyms

Glycerol-3-phosphate phosphatase, G3PP, EC:3.1.3.48, EC:3.1.3.21, AUM

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Validation Data Gallery

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  WB analysis using 25081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25081-1-AP (PGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25081-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25081-1-AP (PGP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 25081-1-AP

  IP result of anti-PGP (IP:25081-1-AP, 4ug; Detection:25081-1-AP 1:600) with Y79 cells lysate 1920ug.

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  IHC staining of human tonsillitis using 25081-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25081-1-AP (PGP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 25081-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25081-1-AP (PGP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 25081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25081-1-AP (PGP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 25081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25081-1-AP (PGP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 25081-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25081-1-AP (PGP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 25081-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25081-1-AP (PGP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 25081-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 25081-1-AP (PGP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 25081-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PGP antibody (25081-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 25081-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PGP (25081-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse heart tissue, K-562 cells, SH-SY5Y cells, Y79 cells
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human tonsillitis tissue, human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

25081-1-AP targets PGP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGP fusion protein Ag18623
• Full Name: phosphoglycolate phosphatase
• Calculated Molecular Weight: 321 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC157035
• Gene Symbol: PGP
• Gene ID (NCBI): 283871
• RRID: AB_2879888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGP(phosphoglycolate phosphatase) belongs to the HAD-like hydrolase superfamily. It is an essential intermediate in the biosynthetic pathway of cardiolipin and a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle. PGP catalyzes the formation of phosphatidylglycerol from phosphatidylglycerophosphate.

Publications

Species	Application	Title
		Acta Biomater; Systematic evaluation of multifunctional paclitaxel-loaded polymeric mixed micelles as a potential anticancer remedy to overcome multidrug resistance.; Authors - Jiulong Zhang
human	IHC	Front Cell Dev Biol; Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.; Authors - Lingyun Zhang
human	WB	Chin Med; The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters; Authors - Wenjie Huang
human	WB,IF	J Nanobiotechnology; Combination of polythyleneimine regulating autophagy prodrug and Mdr1 siRNA for tumor multidrug resistance; Authors - Changduo Wang
human	WB	Eur J Med Chem; Design, synthesis and biological evaluation of novel phenylfuran-bisamide derivatives as P-glycoprotein inhibitors against multidrug resistance in MCF-7/ADR cell; Authors - Zhikun Yang
rat	IF	J Peripher Nerv Syst; Effect of age on metabolomic changes in a model of paclitaxel-induced peripheral neurotoxicity; Authors - Roberta Bonomo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kerstin (Verified Customer) (10-11-2024)

nice detection of PGP in HT1080 WT cells compared to KO cells, few unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HT1080

PGP Antibody Western Blot validation (1:5000 dilution) in HT1080 (Cat no:25081-1-AP)

FH

Yu Siong (Verified Customer) (10-13-2019)

3 protein bands at 150 kDa are of rat liver whole tissue lysate.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat liver tissue

PGP Antibody Western Blot, validation (1:500 dilution) in Rat liver tissue (Cat no:25081-1-AP)