PGK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PGK2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13686-1-AP

Synonyms

PGKPS, PGKB, EC:2.7.2.3

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Validation Data Gallery

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  WB analysis of mouse testis using 13686-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13686-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13686-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13686-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13686-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13686-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse testis using 13686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 13686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells, HepG2 cells, human heart tissue, human testis tissue, mouse brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

13686-1-AP targets PGK2 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGK2 fusion protein Ag4553
• Full Name: phosphoglycerate kinase 2
• Calculated Molecular Weight: 417 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC038843
• Gene Symbol: PGK2
• Gene ID (NCBI): 5232
• RRID: AB_2161237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.

Publications

Species	Application	Title
mouse	WB	Chemosphere; Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.; Authors - Yongli Han
mouse	WB	Cell Physiol Biochem; Effect of Type I Diabetes on the Proteome of Mouse Oocytes.; Authors - Guangjian Jiang
sheep	WB	Theriogenology; Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm.; Authors - Xiaolei Yao
mouse	WB	Nature; A male germ-cell-specific ribosome controls male fertility; Authors - Huiling Li
human	WB	Cell Res; Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate; Authors - Yu-Qing Wu ; KD Validated
mouse	WB,IHC,CoIP	Biomedicines; The Role of Chorein Deficiency in Late Spermatogenesis; Authors - Kaoru Arai