PGD Monoclonal antibody, PBS Only

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  WB analysis using 67916-1-Ig (same clone as 67916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67916-1-Ig (same clone as 67916-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67916-1-Ig (same clone as 67916-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PGD antibody (67916-1-Ig, Clone: 2A10G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

PGD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67916-1-PBS

Clone No.2A10G4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

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Product Information

67916-1-PBS targets PGD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PGD fusion protein Ag6871
• Full Name: phosphogluconate dehydrogenase
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa, 45 kDa
• GenBank Accession Number: BC000368
• Gene Symbol: PGD
• Gene ID (NCBI): 5226
• RRID: AB_2918670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P52209
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)