Pepsinogen II Polyclonal antibody

Pepsinogen II Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16881-1-AP

Synonyms

PGC, PGⅡ, EC:3.4.23.3

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Validation Data Gallery

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  WB analysis of human stomach using 16881-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16881-1-AP (Pepsinogen II antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16881-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 16881-1-AP (Pepsinogen II antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 16881-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16881-1-AP (Pepsinogen II antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16881-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16881-1-AP (Pepsinogen II antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human stomach tissue, mouse stomach tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16881-1-AP targets Pepsinogen II in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pepsinogen II fusion protein Ag10332
• Full Name: progastricsin (pepsinogen C)
• Calculated Molecular Weight: 388 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC073740
• Gene Symbol: PGC
• Gene ID (NCBI): 5225
• RRID: AB_10596919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.