PGAM5 Polyclonal antibody

PGAM5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (4)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 28445-1-AP

Synonyms

BXLBv68, PGAM5

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Validation Data Gallery

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  WB analysis using 28445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28445-1-AP (PGAM5 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28445-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28445-1-AP (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 28445-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PGAM5 antibody (28445-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

28445-1-AP targets PGAM5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, pig, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGAM5 fusion protein Ag28195
• Full Name: phosphoglycerate mutase family member 5
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC008196
• Gene Symbol: PGAM5
• Gene ID (NCBI): 192111
• RRID: AB_2881143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.

Publications

Species	Application	Title
human,mouse	IHC,IF	Cell Biosci; Differential effects of PGAM5 knockout on high fat high fructose diet and methionine choline-deficient diet induced non-alcoholic steatohepatitis (NASH) in mice; Authors - Li Li
human,mouse	WB	Int J Mol Sci; The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.; Authors - Meiyu Cheng
human	WB,IF	Int Immunopharmacol; 4-Octyl itaconate inhibits inflammation to attenuate psoriasis as an agonist of oxeiptosis; Authors - Mengshu You
human,pig	WB,CoIP	J Virol; PGAM5 degrades PDCoV N protein and activates type I interferon to antagonize viral replication; Authors - Xinyu Yang
mouse	WB	Biochim Biophys Acta Mol Cell Res; Tau phosphorylation and OPA1 proteolysis are unrelated events: Implications for Alzheimer's Disease.; Authors - Marcel V Alavi
mouse	WB,IF	Heliyon; LFHP-1c improves cognitive function after TBI in mice by reducing oxidative stress through the PGAM5-NRF2-KEAP1 ternary complex; Authors - Wei Shao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (07-03-2020)

1:1000 dilution in 1%BSA giving a very clear results

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human