PFTK1 Polyclonal antibody

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  WB analysis of HeLa using 21612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21612-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 21612-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21612-1-AP (PFTK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21612-1-AP (PFTK1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21612-1-AP (PFTK1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 21612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21612-1-AP (PFTK1 Antibody) at dilution of 1:50 (under 40x lens).

PFTK1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21612-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

CDK14, hPFTAIRE1, KIAA0834, PFTAIRE protein kinase 1, PFTAIRE1, PFTK1

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, SH-SY5Y cells
• Positive IHC detected in: human kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

21612-1-AP targets PFTK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFTK1 fusion protein Ag16259
• Full Name: PFTAIRE protein kinase 1
• Calculated Molecular Weight: 469 aa, 53 kDa
• Observed Molecular Weight: 48-53 kDa
• GenBank Accession Number: BC136477
• Gene Symbol: PFTK1
• Gene ID (NCBI): 5218
• RRID: AB_10858924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94921
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFTK1 is a Cdc2-related protein kinase, also named as PFTAIRE1, CDK14 and belongs to the CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It acts as a cyclin-dependent kinases that regulates cell cycle progression and cell proliferation. The PFTK1 protein shows high homology with mouse Pftaire1, a Cdc2-related protein expressed primarily in the postnatal and adult nervous system(PMID:9202329). The protein contains 2 predicted nuclear localization signals that the N-terminal nuclear localization signals do not play a role in vivo(PMID:11313143). It has 3 isoforms produced by alternative splicing. This antibody is specific to PFTK1.

Publications

Species	Application	Title
human	WB	Commun Biol; Methylation-modulated PFTK1 regulates gefitinib resistance via Wnt/β-catenin signaling in EGFR mutant non-small-cell lung cancer cells; Authors - Xiaoting Jia
human	WB	Mol Ther Oncolytics; Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus; Authors - Dakota W. Goad
mouse	WB, IHC, IF	Toxicol Lett; CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.; Authors - Daniel Pollack
human	WB	World Neurosurg; MiR-205 Inhibits Sporadic Vestibular Schwannoma Cell Proliferation by Targeting Cyclin-Dependent Kinase 14.; Authors - Xiaoling Yin
human	WB,IHC	Biomed Pharmacother; MiR-542-3p, a microRNA targeting CDK14, suppresses cell proliferation, invasiveness, and tumorigenesis of epithelial ovarian cancer.; Authors - Jingwei Li