PFKM Polyclonal antibody

• Featured Product
• KD/KO Validated

PFKM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30326-1-AP

Synonyms

GSD7, PFK 1, PFK A, PFK M, PFKM, PFKX, Phosphofructokinase 1, Phosphofructokinase M, phosphofructokinase, muscle, Phosphohexokinase

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Validation Data Gallery

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  WB analysis using 30326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30326-1-AP (PFKM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 30326-1-AP

  WB result of PFKM antibody (30326-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HEK-293 cells.

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  WB analysis using 30326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30326-1-AP (PFKM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 30326-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30326-1-AP (PFKM antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 30326-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30326-1-AP (PFKM antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30326-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed 48-well HepG2 cells using PFKM antibody (30326-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, HEK-293 cells, Raji cells, MCF-7 cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 48-well HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30326-1-AP targets PFKM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFKM fusion protein Ag30840
• Full Name: phosphofructokinase, muscle
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC021203
• Gene Symbol: PFKM
• Gene ID (NCBI): 5213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and Two domains subfamily. PFKM catalyze the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular mass of 85, 82 and 93 kDa. The antibody is specific to PFKM.