PFKL Polyclonal antibody

PFKL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15652-1-AP

Synonyms

PFK B, PFKL, Phosphofructokinase 1, phosphofructokinase, liver, Phosphohexokinase

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Validation Data Gallery

• 

  WB analysis of HeLa using 15652-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15652-1-AP (PFKL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15652-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15652-1-AP (PFKL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15652-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15652-1-AP (PFKL antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

15652-1-AP targets PFKL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFKL fusion protein Ag8250
• Full Name: phosphofructokinase, liver
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC008964
• Gene Symbol: PFKL
• Gene ID (NCBI): 5211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKL(6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK)(ATP:D-fructose-6-phosphate-1-phosphotransferase).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings,PFK exsits as a system of three of subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs(PMID: 2139864). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.; Authors - Shaoxun Xiang
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB	Cancer Res; Glucose catabolism in liver tumors induced by c-MYC can be sustained by various PKM1/PKM2 ratios and pyruvate kinase activities.; Authors - Andrés Méndez-Lucas
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong
human	WB	Oncotarget; The platelet isoform of phosphofructokinase contributes to metabolic reprogramming and maintains cell proliferation in clear cell renal cell carcinoma.; Authors - Jun Wang
human	WB	Int J Oncol; lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma.; Authors - Xiao Li