PFAS Recombinant antibody

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  WB analysis using 83074-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83074-2-RR (PFAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83074-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83074-2-RR against Human PFAS were performed. The affinity constant is 0.387 nM.

PFAS {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83074-2-RR

Clone No.230422D11

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

FGAMS, FGARAT, KIAA0361, PFAS, PFAS protein, PURL

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, Raji cells, HEK-293T cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83074-2-RR targets PFAS in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PFAS fusion protein Ag20162
• Full Name: phosphoribosylformylglycinamidine synthase
• Calculated Molecular Weight: 1338 aa, 145 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC167158
• Gene Symbol: PFAS
• Gene ID (NCBI): 5198
• RRID: AB_3670793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15067
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.