PFAS Polyclonal antibody

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  WB analysis using 24716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24716-1-AP (PFAS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 24716-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24716-1-AP (PFAS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 24716-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PFAS antibody (24716-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PFAS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24716-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FGAR-AT, FGARAT, FGAR amidotransferase, FGAMS, FGAM synthase

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24716-1-AP targets PFAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFAS fusion protein Ag20162
• Full Name: phosphoribosylformylglycinamidine synthase
• Calculated Molecular Weight: 1338 aa, 145 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC167158
• Gene Symbol: PFAS
• Gene ID (NCBI): 5198
• RRID: AB_3669446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15067
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.