PF4 Polyclonal antibody

PF4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21157-1-AP

Synonyms

C X C motif chemokine 4, CXCL4, Iroplact, Oncostatin A, PF 4, PF4, platelet factor 4, SCYB4



Tested Applications

Positive WB detected inHuman peripheral blood platelets tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21157-1-AP targets PF4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PF4 fusion protein Ag12345
Full Name platelet factor 4
Calculated Molecular Weight 101 aa, 11 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC093965
Gene Symbol PF4
Gene ID (NCBI) 5196
RRIDAB_2878819
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation

Protocols

Product Specific Protocols
WB protocol for PF4 antibody 21157-1-APDownload protocol
IHC protocol for PF4 antibody 21157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

J Virol

Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage.

Authors - Zhichao Pei
humanIF

Cell Biosci

Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis.

Authors - Jiaqi Jin
humanWB

Amyloid

Binding of serum-derived amyloid-associated proteins to amyloid fibrils

Authors - Yohei Misumi
humanWB,IF

J Vasc Access

Functions for platelet factor 4 (PF4/CXCL4) and its receptors in fibroblast-myofibroblast transition and fibrotic failure of arteriovenous fistulas (AVFs)

Authors - Yuxuan Xiao