PEX5L Polyclonal antibody

PEX5L Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13084-1-AP

Synonyms

Peroxin 5 related protein, PEX2 related protein, PEX5 like protein, PEX5 related protein, PEX5L, PEX5R, Pex5Rp, PXR2, PXR2B

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Validation Data Gallery

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  WB analysis of mouse brain using 13084-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13084-1-AP (PEX5L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 13084-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13084-1-AP (PEX5L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13084-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13084-1-AP (PEX5L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13084-1-AP

  IP result of anti-PEX5L (IP:13084-1-AP, 4ug; Detection:13084-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 13084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13084-1-AP (PEX5L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13084-1-AP (PEX5L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

13084-1-AP targets PEX5L in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX5L fusion protein Ag3749
• Full Name: peroxisomal biogenesis factor 5-like
• Calculated Molecular Weight: 626 aa, 70 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC036183
• Gene Symbol: PEX5L
• Gene ID (NCBI): 51555
• RRID: AB_2877914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	Mol Psychiatry; Glucocorticoid-glucocorticoid receptor-HCN1 channels reduce neuronal excitability in dorsal hippocampal CA1 neurons.; Authors - Jiwon Kim