PEX5 Polyclonal antibody

• Featured Product
• KD/KO Validated

PEX5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 12545-1-AP

Synonyms

PTS1 BP, PEX, Peroxisome receptor 1, Peroxisomal C-terminal targeting signal import receptor, Peroxin-5

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Validation Data Gallery

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  WB analysis of HEK-293 using 12545-1-AP

  WB result of PEX5 antibody (12545-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX5 transfected HEK-293 cells.

• 

  WB analysis of human brain using 12545-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12545-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12545-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12545-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12545-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12545-1-AP

  IP result of anti-PEX5 (IP:12545-1-AP, 4ug; Detection:12545-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human kidney using 12545-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12545-1-AP (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12545-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12545-1-AP (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 12545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12545-1-AP (PEX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12545-1-AP (PEX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12545-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12545-1-AP (PEX5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 12545-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug PEX5 Polyclonal antibody (12545-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human brain tissue, L02 cells, mouse kidney tissue, mouse brain tissue, human kidney tissue, HEK-293 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

12545-1-AP targets PEX5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX5 fusion protein Ag3268
• Full Name: peroxisomal biogenesis factor 5
• Calculated Molecular Weight: 631 aa, 70 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC010621
• Gene Symbol: PEX5
• Gene ID (NCBI): 5830
• RRID: AB_2268102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder. This antibody recognizes endogenous PEX5, which migrates with an apparent molecular mass of 80 kDa (PMID: 7790377).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; A tuberous sclerosis complex signalling node at the peroxisome regulates mTORC1 and autophagy in response to ROS.; Authors - Zhang Jiangwei J ; KD Validated
human	WB, IF	Nat Cell Biol; ATM functions at the peroxisome to induce pexophagy in response to ROS.; Authors - Jiangwei Zhang
human	WB,IF,IP	Autophagy; Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.; Authors - Michael S Dahabieh
human	WB,IF	Cell Mol Life Sci; Dietary cysteine and methionine promote peroxisome elevation and fat loss by induction of CG33474 expression in Drosophila adipose tissue; Authors - Meng Liu
human	WB	Hum Mol Genet; A novel type of rhizomelic chondrodysplasia punctata, RCDP5, is caused by loss of the PEX5 long isoform.; Authors - Tuva Barøy
		Biochim Biophys Acta Mol Cell Biol Lipids; Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes.; Authors - Zhihui Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhihui (Verified Customer) (12-12-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T And mouse astrocytes

PEX5 Antibody Western Blot, validation (1:1000 dilution) in HEK293T And mouse astrocytes (Cat no:12545-1-AP)

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