PEX16 Monoclonal antibody, PBS Only

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  WB analysis using 68261-1-Ig (same clone as 68261-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68261-1-Ig (PEX16 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68261-1-PBS in a different storage buffer formulation.

PEX16 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68261-1-PBS

Clone No.2D7G4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, Indirect ELISA

Synonyms

Peroxin 16, PEX16

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Product Information

68261-1-PBS targets PEX16 in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6574

  Product name: Recombinant human PEX16 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 132-346 aa of BC000467

  Sequence: KAGLQTSPPIVPLDRETQAQPPDGDHSPGNHEQSYVGKRSNRVVRTLQNTPSLHSRHWGAPQQREGRQQQHHEELSATPTPLGLQETIAEFLYIARPLLHLLSLGLWGQRSWKPWLLAGVVDVTSLSLLSDRKGLTRRERRELRRRTILLLYYLLRSPFYDRFSEARILFLLQLLADHVPGVGLVTTSQRAASPCLPARPHTQPWSPPAFLPGHP

• Full Name: peroxisomal biogenesis factor 16
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000467
• Gene Symbol: PEX16
• Gene ID (NCBI): 9409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y5Y5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).