Biotin-conjugated PEX14 Polyclonal antibody

• Featured Product
• KD/KO Validated

PEX14 Polyclonal Antibody for IHC, IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IHC, IF/ICC

Conjugate

Biotin

Cat no : Biotin-10594

Synonyms

PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14

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Validation Data Gallery

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  IHC staining of mouse liver using Biotin-10594

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using Biotin-10594 (PEX14 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using Biotin-10594

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using Biotin-10594 (PEX14 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using Biotin-10594

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Biotin PEX14 antibody (Biotin-10594) at dilution of 1:200 and Alexa Fluor 594 Streptavidin.

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Tested Applications

• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-10594 targets PEX14 in IHC, IF/ICC applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX14 fusion protein Ag0932
• Full Name: peroxisomal biogenesis factor 14
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006327
• Gene Symbol: PEX14
• Gene ID (NCBI): 5195
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.