PEX13 Polyclonal antibody

PEX13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26649-1-AP

Synonyms

NALD, Peroxin 13, PEX13, ZWS

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Validation Data Gallery

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  WB analysis of Jurkat using 26649-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26649-1-AP (PEX13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26649-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26649-1-AP (PEX13 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IHC staining of human kidney using 26649-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26649-1-AP (PEX13 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26649-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26649-1-AP (PEX13 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 26649-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26649-1-AP (PEX13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 26649-1-AP is PEX13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX13 fusion protein Ag24400
• Full Name: peroxisomal biogenesis factor 13
• Calculated Molecular Weight: 403 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC067090
• Gene Symbol: PEX13
• Gene ID (NCBI): 5194
• RRID: AB_2880588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Autophagy; Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.; Authors - Michael S Dahabieh
human	WB	Biol Chem; Comparison of human PEX knockout cell lines suggests a dual role of PEX1 in peroxisome biogenesis; Authors - Julia Ott
human	WB	Nat Commun; Modulation of peroxisomal import by the PEX13 SH3 domain and a proximal FxxxF binding motif; Authors - Stefan Gaussmann