PEX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PEX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 13669-1-AP

Synonyms

PEX, Peroxisome biogenesis factor 1, Peroxisomal ATPase PEX1, Peroxin-1, Peroxin 1

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Validation Data Gallery

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  WB analysis of Jurkat using 13669-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13669-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 13669-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13669-1-AP

  Immunofluorescent analysis of HeLa cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 13669-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

13669-1-AP targets PEX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX1 fusion protein Ag4623
• Full Name: peroxisomal biogenesis factor 1
• Calculated Molecular Weight: 1283 aa, 143 kDa
• Observed Molecular Weight: 143 kDa
• GenBank Accession Number: BC035575
• Gene Symbol: PEX1
• Gene ID (NCBI): 5189
• RRID: AB_2162104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	IF	Autophagy; Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.; Authors - Marcus Nordgren
human	WB,IF	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
mouse	WB	Cell Commun Signal; TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions; Authors - Laxman Manandhar
human	IHC	Eur J Hum Genet; Spectrum of PEX1 and PEX6 variants in Heimler syndrome.; Authors - Claire E L Smith
human	WB	Mol Ther Methods Clin Dev; AAV-mediated PEX1 gene augmentation improves visual function in the PEX1-Gly844Asp mouse model for mild Zellweger spectrum disorder.; Authors - Catherine Argyriou