PEX1 Polyclonal antibody

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  WB analysis of Jurkat using 13669-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13669-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 13669-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13669-1-AP

  Immunofluorescent analysis of HeLa cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 13669-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PEX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13669-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.6.4.-, Peroxin 1, Peroxin-1, Peroxisomal ATPase PEX1, Peroxisome biogenesis factor 1

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

13669-1-AP targets PEX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX1 fusion protein Ag4623
• Full Name: peroxisomal biogenesis factor 1
• Calculated Molecular Weight: 1283 aa, 143 kDa
• Observed Molecular Weight: 143 kDa
• GenBank Accession Number: BC035575
• Gene Symbol: PEX1
• Gene ID (NCBI): 5189
• RRID: AB_2162104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43933
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxin (PEX) proteins generate and maintain peroxisomes. The peroxin-1 (PEX1) ATPase facilitates the recycling of the peroxisome matrix protein receptor PEX5 and is the most commonly affected peroxin in human peroxisome biogenesis disorders (PMID: 28600347).  PEX1 and PEX6 are the only members of the AAA family (for ATPases associated with diverse cellular) activities implicated in peroxisome biogenesis and are closely related to p97, which functions in endoplasmic reticulum-associated protein degradation to retrotranslocate endoplasmic reticulum proteins to the cytosol (PMID: 9695811; 11740563).

Publications

Species	Application	Title
	IF	Autophagy; Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.; Authors - Marcus Nordgren
human	WB,IF	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
mouse	WB	Cell Commun Signal; TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions; Authors - Laxman Manandhar
human	IHC	Eur J Hum Genet; Spectrum of PEX1 and PEX6 variants in Heimler syndrome.; Authors - Claire E L Smith
human	WB	Mol Ther Methods Clin Dev; AAV-mediated PEX1 gene augmentation improves visual function in the PEX1-Gly844Asp mouse model for mild Zellweger spectrum disorder.; Authors - Catherine Argyriou