PERK/EIF2AK3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PERK/EIF2AK3 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 24390-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

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  WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24390-1-AP (same clone as 24390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 10x lens).. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24390-1-AP (same clone as 24390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 40x lens).. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 24390-1-AP (same clone as 24390-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PERK/EIF2AK3 (24390-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

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Product Information

24390-1-PBS targets PERK/EIF2AK3 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PERK/EIF2AK3 fusion protein Ag18254
• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated Molecular Weight: 1116 aa, 125 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC126354
• Gene Symbol: PERK
• Gene ID (NCBI): 9451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).