PER2 Polyclonal antibody

PER2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29954-1-AP

Synonyms

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)



Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IF/ICC detected inMDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29954-1-AP targets PER2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PER2 fusion protein Ag31471
Full Name period homolog 2 (Drosophila)
Calculated Molecular Weight137KD
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_022817
Gene Symbol PER2
Gene ID (NCBI) 8864
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774). 29954-1-AP detected molecular weight of 45 kDa.

Protocols

Product Specific Protocols
WB protocol for PER2 antibody 29954-1-APDownload protocol
IF protocol for PER2 antibody 29954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols