PER2 Polyclonal antibody

PER2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29954-1-AP

Synonyms

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)

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Validation Data Gallery

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  WB analysis using 29954-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29954-1-AP (PER2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of MDA-MB-231 using 29954-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using PER2 antibody (29954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells
• Positive IF/ICC detected in: MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29954-1-AP targets PER2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PER2 fusion protein Ag31471
• Full Name: period homolog 2 (Drosophila)
• Calculated Molecular Weight: 137KD
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_022817
• Gene Symbol: PER2
• Gene ID (NCBI): 8864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774). 29954-1-AP detected molecular weight of 45 kDa.