PER2 Polyclonal antibody

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  WB analysis of BxPC-3 using 20359-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20359-1-AP (PER2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 20359-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20359-1-AP (PER2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20359-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20359-1-AP (PER2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20359-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20359-1-AP (PER2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20359-1-AP

  IP result of anti-PER2 (IP:20359-1-AP, 3ug; Detection:20359-1-AP 1:300) with HEK-293 cells lysate 1000ug.

PER2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 20359-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)

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Tested Applications

• Positive WB detected in: BxPC-3 cells, Y79 cells, K-562 cells, L02 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

20359-1-AP targets PER2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: period homolog 2 (Drosophila)
• Calculated Molecular Weight: 137 kDa
• Observed Molecular Weight: 136 kDa
• GenBank Accession Number: NM_022817
• Gene Symbol: PER2
• Gene ID (NCBI): 8864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15055
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. PER2 is a negative element in the circadian transcriptional loop. PER2 influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. It negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. The antibody is specific to PER2. It can recognize isoform1 and isoform2 of PER2.

Publications

Species	Application	Title
human	WB	Cell; Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR.; Authors - Zandra E Walton
human	WB	Cell Metab; MYC Disrupts the Circadian Clock and Metabolism in Cancer Cells.; Authors - Brian J Altman
human	WB	Sci Adv; Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.; Authors - Yool Lee
human	WB	Nat Commun; Correspondence: Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα.; Authors - Brian J Altman
human	WB, IF, IP	Elife; KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.; Authors - Yool Lee
mouse	WB	Elife; NRF2 regulates core and stabilizing circadian clock loops, coupling redox and timekeeping inMus musculus.; Authors - Ryan S Wible