PER1 Polyclonal antibody

PER1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 13463-1-AP

Synonyms

RIGUI, period homolog 1 (Drosophila), PER, KIAA0482, hPER1

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Validation Data Gallery

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  WB analysis of A549 using 13463-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13463-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13463-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13463-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13463-1-AP (PER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 13463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13463-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13463-1-AP (PER1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13463-1-AP

  Immunofluorescent analysis of Hela cells, using PER1 antibody 13463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, mouse kidney tissue, mouse liver tissue
• Positive IHC detected in: human breast cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

13463-1-AP targets PER1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PER1 fusion protein Ag4249
• Full Name: period homolog 1 (Drosophila)
• Calculated Molecular Weight: 1290 aa, 136 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC028207
• Gene Symbol: PER1
• Gene ID (NCBI): 5187
• RRID: AB_2161536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PER1 is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation [PMID: 21514639]. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus [PMID: 9856465, 20930143]. In addition to bands of the predicted molecular mass of 140 kDa, additional PER1 species of 55 and 45 kDa is detected that is predominantly nuclear [PMID: 11726537] .

Publications

Species	Application	Title
		Cell Metab; Dysfunctional circadian clock accelerates cancer metastasis by intestinal microbiota triggering accumulation of myeloid-derived suppressor cells; Authors - Jing-Lin Liu
mouse	WB	Nat Commun; Angptl8 mediates food-driven resetting of hepatic circadian clock in mice.; Authors - Siyu Chen
human	IF	Theranostics; Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model.; Authors - Mark A Naven
human	IHC	J Transl Med; Prognostic risk factors of serous ovarian carcinoma based on mesenchymal stem cell phenotype and guidance for therapeutic efficacy; Authors - Xiaohui Yang
mouse	WB	Int J Mol Sci; Uncovering the Novel Role of NR1D1 in Regulating BNIP3-Mediated Mitophagy in Ulcerative Colitis; Authors - Yidong Chen
human	WB	J Agric Food Chem; Capsaicin Attenuates Oleic Acid-Induced Lipid Accumulation via the Regulation of Circadian Clock Genes in HepG2 Cells.; Authors - Run Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-05-2023)

The antibody works well. I have been using this antibody for human cardiomyocytes, human keratinocytes cell lines, and mouse tissues such as the liver and skin.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin