Twinkle/PEO1 Polyclonal antibody

Twinkle/PEO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18793-1-AP

Synonyms

ATXN8, C10orf2, IOSCA, PEO, PEO1, PEOA3, SANDO, SCA8, Twinkle, Twinkle protein, mitochondrial, TWINL

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Validation Data Gallery

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  WB analysis using 18793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18793-1-AP (Twinkle antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 18793-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50.

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  IHC staining of human skeletal muscle using 18793-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 18793-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 18793-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18793-1-AP (Twinkle antibody at dilution of 1:50.

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Tested Applications

• Positive WB detected in: Daudi cells, MCF-7 cells, K-562 cells, HeLa cells
• Positive IHC detected in: human pancreas tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18793-1-AP targets Twinkle/PEO1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Twinkle/PEO1 fusion protein Ag6628
• Full Name: chromosome 10 open reading frame 2
• Calculated Molecular Weight: 684 aa, 77 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC033762
• Gene Symbol: PEO1
• Gene ID (NCBI): 56652
• RRID: AB_2066354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Twinkle, also named as Progressive external ophthalmoplegia 1 protein is a 684 amino acid protein, which involves in mitochondrial DNA (mtDNA) metabolism. The predicted full-length protein is 684 amino acids, with amolecular mass of 77 kD, whereas the splice variant mRNAencodes a 66-kD product of 582 amino acids, lacking residues 579-684 and terminating with four unique amino acids (PMID: 11431692).