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PEMT Recombinant antibody, PBS Only

PEMT Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242150D5

Cat no : 85099-1-PBS

Synonyms

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using PEMT Recombinant antibody (85099-1-RR)

    WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    WB analysis of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

Product Information

85099-1-PBS targets PEMT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEMT fusion protein Ag8034
Full Name phosphatidylethanolamine N-methyltransferase
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC050593
Gene Symbol PEMT
Gene ID (NCBI) 10400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).