Serpin F1/PEDF Monoclonal antibody, PBS Only

• 

  WB analysis of human plasma using 66554-1-Ig (same clone as 66554-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66554-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig plasma using 66554-1-Ig (same clone as 66554-1-PBS)

  pig plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66554-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 66554-1-Ig (same clone as 66554-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66554-1-PBS in a different storage buffer formulation.

Serpin F1/PEDF Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66554-1-PBS

Clone No.2C5C8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, Indirect ELISA

Synonyms

PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein

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Product Information

66554-1-PBS targets Serpin F1/PEDF in WB, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Serpin F1/PEDF fusion protein Ag26660
• Full Name: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• Calculated Molecular Weight: 418 aa, 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC013984
• Gene Symbol: PEDF
• Gene ID (NCBI): 5176
• RRID: AB_2881916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P36955
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.