Serpin F1/PEDF Polyclonal antibody

Serpin F1/PEDF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 26045-1-AP

Synonyms

PIG35, PEDF, Pigment epithelium-derived factor, EPC-1, EPC 1

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Validation Data Gallery

• 

  WB analysis of human serum using 26045-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 26045-1-AP (PEDF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 26045-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 26045-1-AP (PEDF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 26045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26045-1-AP (PEDF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A375 cells, human serum
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

26045-1-AP targets Serpin F1/PEDF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Serpin F1/PEDF fusion protein Ag23296
• Full Name: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• Calculated Molecular Weight: 418 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC013984
• Gene Symbol: PEDF
• Gene ID (NCBI): 5176
• RRID: AB_2880349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

Publications

Species	Application	Title
human	WB,IF	J Cell Mol Med; Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization; Authors - Lele Li
human	WB	Hum Gene Ther; Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor.; Authors - Hao Zhang
human	WB	Cell Death Dis; TFAP2B overexpression contributes to tumor growth and progression of thyroid cancer through the COX-2 signaling pathway.; Authors - Xiaoyan Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (05-14-2021)

very good for WB

• Applications: Western Blot