PECR Polyclonal antibody

• Featured Product
• KD/KO Validated

PECR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14901-1-AP

Synonyms

DCR RP, HPDHase, HSA250303, PECR, pVI ARL, SDR29C1, TERP

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Validation Data Gallery

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  WB analysis using 14901-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14901-1-AP (PECR antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14901-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14901-1-AP (PECR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14901-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14901-1-AP (PECR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14901-1-AP

  IP result of anti-PECR (IP:14901-1-AP, 4ug; Detection:14901-1-AP 1:2000) with mouse liver tissue lysate 3040 ug.

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  IHC staining of human liver cancer using 14901-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14901-1-AP (PECR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14901-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14901-1-AP (PECR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, human testis tissue, human kidney tissue, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

14901-1-AP targets PECR in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PECR fusion protein Ag6693
• Full Name: peroxisomal trans-2-enoyl-CoA reductase
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC002529
• Gene Symbol: PECR
• Gene ID (NCBI): 55825
• RRID: AB_2161166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisomal trans-2-enoyl-CoA reductase (PECR) is also called TERP and TECR, which is located on chromosome q35 with 86627 bases. It can participate in carbon chain elongation in fatty acid metabolism and catalyze the last reaction of four long chain fatty acid elongation cycles. Each cycle of PECR adds two carbons to the long chain and very long chain fatty acid (VLCFA) chains, reducing the intermediate of trans-2,3-enoyl coenzyme A fatty acid to acyl coenzyme A, which can further extend the carbon chain by entering a new elongation cycle. Meanwhile, PECR is a peroxisome protein involved in fatty acid synthesis and plays an important role in milk fat synthesis. The molecular mass of PECR is 33 kDa. (PMID: 31467878)

Publications

Species	Application	Title
human	WB,IHC	Acta Histochem; Peroxisomal trans-2-enoyl-CoA inhibits proliferation, migration and invasion of hepatocellular carcinoma cells; Authors - Qingqing Luo ; KD Validated
human	WB	bioRxiv; The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy; Authors - Iris D Montes