PECI Polyclonal antibody

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  WB analysis of human placenta using 20383-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20383-1-AP (PECI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 20383-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20383-1-AP (PECI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 20383-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20383-1-AP (PECI antibody) at dilution of 1:100 (under 40x lens).

PECI Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20383-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ACBD2, D3,D2 enoyl CoA isomerase, DBI related protein 1, Dodecenoyl CoA isomerase, DRS 1, DRS1, ECI2, HCA88, KIAA0536, PECI

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human heart tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20383-1-AP targets PECI in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PECI fusion protein Ag14211
• Full Name: peroxisomal D3,D2-enoyl-CoA isomerase
• Calculated Molecular Weight: 394 aa, 44 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC034702
• Gene Symbol: PECI
• Gene ID (NCBI): 10455
• RRID: AB_10696313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75521
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	ACS Omega; Proteomics Analysis Provides Insights into the Role of Lipid Metabolism in T2DM-Related Sarcopenia; Authors - Jingying Wu