PEBP4 Recombinant antibody

• 

  IF Staining of A549 using 83589-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PEBP4 antibody (83589-5-RR, Clone: 240547D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83589-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83589-5-RR against Human PEBP4 were performed. The affinity constant is below 1 pM.

PEBP4 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Cat No. 83589-5-RR

Clone No.240547D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

PEBP 4, hPEBP4, GWTM1933, CORK1, CORK 1

---

Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83589-5-RR targets PEBP4 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PEBP4 fusion protein Ag9710
• Full Name: phosphatidylethanolamine-binding protein 4
• Calculated Molecular Weight: 223 aa, 25 kDa
• GenBank Accession Number: BC020779
• Gene Symbol: PEBP4
• Gene ID (NCBI): 157310
• RRID: AB_3671203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96S96
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEBP4 (Phosphatidylethanolamine binding protein 4) is a multifunctional protein that belongs to a small family (PEBP1-4) in mammalian cells. PEBP4 is evolutionally divergent from the other three forms that share almost 80% identity and more than 90% similarity, suggesting that PEBP4 is functionally different from other forms. At cellular levels, it has been shown that the knockdown of PEBP4 in cancer cells suppresses cell proliferation, migration, and invasion, leading to their apoptosis. In contrast, overexpression of PEBP4 induces opposite changes and confers resistance to chemotherapy and radiotherapy (PMID: 35955931).