PDZK1 Polyclonal antibody

PDZK1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10507-2-AP

Synonyms

CAP70, CLAMP, NaPi Cap1, NHERF 3, NHERF3, PDZ domain containing 1, PDZD1, PDZK1

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Validation Data Gallery

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  WB analysis of mouse kidney using 10507-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10507-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10507-2-AP

  IP result of anti-PDZK1 (IP:10507-2-AP, 3ug; Detection:10507-2-AP 1:500) with HEK-293 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• CoIP: See 1 publications below

Product Information

10507-2-AP targets PDZK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDZK1 fusion protein Ag0787
• Full Name: PDZ domain containing 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC006496
• Gene Symbol: PDZK1
• Gene ID (NCBI): 5174
• RRID: AB_2299495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncotarget; Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.; Authors - Yi Huo
human	IHC	Mol Med; PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth.; Authors - Kim Hogyoung H
human	IHC	Mol Med; Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37.; Authors - Hogyoung Kim
human	WB,IHC	Cell Div; miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism; Authors - Huihui Gao ; KD Validated
human	WB,IHC,CoIP	Aging (Albany NY); Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1; Authors - Xing Ren ; KD Validated
human,mouse	WB,IHC	Bone Res; PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function; Authors - Yan Shao