PDZD2 Polyclonal antibody

PDZD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30191-1-AP

Synonyms

AIPC, KIAA0300, PAPIN, PDZ domain containing 2, PDZD2, PDZK3, PIN1

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Validation Data Gallery

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  WB analysis using 30191-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30191-1-AP (PDZD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 30191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30191-1-AP (PDZD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 30191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30191-1-AP (PDZD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30191-1-AP targets PDZD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDZD2 fusion protein Ag32900
• Full Name: PDZ domain containing 2
• Calculated Molecular Weight: 302 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: NM_178140
• Gene Symbol: PDZD2
• Gene ID (NCBI): 23037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDZD2, PDZ-domain containing-2, also named as AIPC, KIAA0300 and PDZK3, is associated with the early promotion of prostate tumoregenesis. The antibody recognizes near the N-term of PDZD2. PDZD2 is a novel factor that affects the growth and differentiation of human fetal pancreatic progenitor cells. The secreted form sPDZD2 can be detected 37 kDa (PMID: 18037333).