PDX1 Polyclonal antibody

PDX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 20989-1-AP

Synonyms

Glucose sensitive factor, GSF, IDX 1, IPF 1, IPF1, Islet/duodenum homeobox 1, IUF 1, IUF1, MODY4, PDX 1, PDX1, STF 1

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Validation Data Gallery

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  WB analysis of BxPC-3 using 20989-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20989-1-AP (PDX1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20989-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20989-1-AP (PDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below
• IF: See 3 publications below

Product Information

20989-1-AP targets PDX1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: pancreatic and duodenal homeobox 1
• Calculated molecular weight: 31 kDa
• Observed molecular weight: 40-50 kDa
• GenBank accession number: NM_000209
• Gene symbol: PDX1
• Gene ID (NCBI): 3651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pancreatic duodenal homeobox-1 protein (PDX-1), also designated INS promoter factor (IPF1), INS upstream factor 1 (IUF1), somatostatin trans-activating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic beta-cells. PDX-1 is a key regulator of pancreatic islet development and INS gene transcription in beta-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3b, HNF-1a and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of beta-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis.

Publications

Species	Application	Title
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang
mouse	IF	Biofabrication; Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells.; Authors - Yanrong Yu
mouse,rat	WB	J Biol Chem; Tectorigenin enhances PDX1 expression and protects pancreatic β-cells by activating ERK and reducing ER stress.; Authors - Xinlei Yao
mouse	WB	J Biol Chem; PMID: 29259128; Authors - Hang Li
rat	IF	Front Neurosci; Effects of Neuropeptide Substance P on Proliferation and β-Cell Differentiation of Adult Pancreatic Ductal Cells.; Authors - Nan Zhang
mouse	WB	Pharm Biol; Screening active components from Rubus amabilis for pancreatic β-cells protection.; Authors - Min Sun