PDX1 Polyclonal antibody

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• PDX1 Polyclonal antibody (20989-1-AP)

PDX1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20989-1-AP Copied!

Publications (30)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Glucose sensitive factor, Glucose-sensitive factor, GSF, IDX 1, IDX-1

• 

  WB analysis of BxPC-3 using 20989-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20989-1-AP (PDX1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 20989-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20989-1-AP (PDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 20989-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20989-1-AP (PDX1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-3 cells, BxPC-3 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20989-1-AP targets PDX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: pancreatic and duodenal homeobox 1
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: NM_000209
• Gene Symbol: PDX1
• Gene ID (NCBI): 3651
• RRID: AB_2878784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P52945
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pancreatic duodenal homeobox-1 protein (PDX-1), also designated INS promoter factor (IPF1), INS upstream factor 1 (IUF1), somatostatin trans-activating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic beta-cells. PDX-1 is a key regulator of pancreatic islet development and INS gene transcription in beta-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3b, HNF-1a and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of beta-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis.

Publications

What published studies show

The PDX1 Polyclonal antibody (Cat# 20989-1-AP) has 30 citations published in journals including Adv Sci, Biomaterials, J Biol Chem, Diabetes Obes Metab, ACS Appl Mater Interfaces, Sci Rep, Front Pharmacol, Biochem Pharmacol, and Oncotarget. Research fields span pancreatic β-cell biology, type 1 and type 2 diabetes, islet differentiation and dedifferentiation, oxidative stress, regenerative medicine, stem cell therapy, gut microbiome, and cancer (prostate, glioblastoma).

Automatically generated from the 30 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Adv Sci (Weinh); Unraveling the Role of MDK-SDC4 Interaction in Pancreatic Cancer-Associated New-Onset Diabetes by Single-Cell Transcriptomic Analysis; Authors - Zengyu Feng
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang
mouse	IF	Biofabrication; Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells.; Authors - Yanrong Yu
rat	WB	Antioxidants (Basel); Single Low-Dose Ionizing Radiation Transiently Enhances Rat RIN-m5F Cell Function via the ROS/p38 MAPK Pathway Without Inducing Cell Damage; Authors - Jitai Zhang
rat	WB	Antioxidants (Basel); Glutathione Induces Keap1 S-Glutathionylation and Mitigates Oscillating Glucose-Induced β-Cell Dysfunction by Activating Nrf2; Authors - Xiufang Chen
mouse	WB	ACS Appl Mater Interfaces; Targeted Polymeric Nanoparticles Based on Mangiferin for Enhanced Protection of Pancreatic β-Cells and Type 1 Diabetes Mellitus Efficacy.; Authors - Mengdi Wang