PDX1 Polyclonal antibody

PDX1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20989-1-AP

Synonyms

Glucose sensitive factor, GSF, IDX 1, IPF 1, IPF1, Islet/duodenum homeobox 1, IUF 1, IUF1, MODY4, PDX 1, PDX1, STF 1



Tested Applications

Positive WB detected inPC-3 cells, BxPC-3 cells
Positive IF detected inhuman pancreas cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20989-1-AP targets PDX1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name pancreatic and duodenal homeobox 1
Calculated molecular weight 31 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberNM_000209
Gene symbol PDX1
Gene ID (NCBI) 3651
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pancreatic duodenal homeobox-1 protein (PDX-1), also designated INS promoter factor (IPF1), INS upstream factor 1 (IUF1), somatostatin trans-activating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic beta-cells. PDX-1 is a key regulator of pancreatic islet development and INS gene transcription in beta-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3b, HNF-1a and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of beta-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis.

Protocols

Product Specific Protocols
WB protocol for PDX1 antibody 20989-1-APDownload protocol
IF protocol for PDX1 antibody 20989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Appl Mater Interfaces

Targeted Polymeric Nanoparticles Based on Mangiferin for Enhanced Protection of Pancreatic β-Cells and Type 1 Diabetes Mellitus Efficacy.

Authors - Mengdi Wang
ratWB,IF

Cell Reprogram

PMID: 29303357

Authors - Ying Luo
mouseWB

Pharm Biol

Screening active components from Rubus amabilis for pancreatic β-cells protection.

Authors - Min Sun
ratIF

Front Neurosci

Effects of Neuropeptide Substance P on Proliferation and β-Cell Differentiation of Adult Pancreatic Ductal Cells.

Authors - Nan Zhang
mouseWB

J Biol Chem

PMID: 29259128

Authors - Hang Li
mouse,ratWB

J Biol Chem

Tectorigenin enhances PDX1 expression and protects pancreatic β-cells by activating ERK and reducing ER stress.

Authors - Xinlei Yao