Podoplanin Polyclonal antibody

Podoplanin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22099-1-AP

Synonyms

AGGRUS, D2 40, D2 40,M2A, D240, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, Podoplanin, PSEC0025, T1 alpha, T1A, T1A 2

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Validation Data Gallery

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  WB analysis of human brain using 22099-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22099-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 22099-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 22099-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 22099-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22099-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22099-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, human placenta tissue
• Positive IHC detected in: human appendicitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22099-1-AP is Podoplanin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Podoplanin fusion protein Ag17307
• Full Name: podoplanin
• Calculated Molecular Weight: 238 aa, 25 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC022812
• Gene Symbol: Podoplanin
• Gene ID (NCBI): 10630
• RRID: AB_2878995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.