PDK4 Recombinant antibody

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  WB analysis of mouse kidney using 83583-1-RR

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 83583-1-RR (PDK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 83583-1-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83583-1-RR (PDK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 83583-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83583-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83583-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 83583-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 83583-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDK4 Recombinant antibody (83583-1-RR, Clone:240551E4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83583-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83583-1-RR against Human PDK4 were performed. The affinity constant is below 1 pM.

PDK4 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83583-1-RR

Clone No.240551E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

PDHK4, EC:2.7.11.2, 240551E4, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse lung tissue
• Positive IHC detected in: mouse skeletal muscle tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83583-1-RR targets PDK4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDK4 fusion protein Ag3629
• Full Name: pyruvate dehydrogenase kinase, isozyme 4
• Calculated Molecular Weight: 411 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC040239
• Gene Symbol: PDK4
• Gene ID (NCBI): 5166
• RRID: AB_3671197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16654
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).