PDK3 Polyclonal antibody

PDK3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12215-1-AP

Synonyms

PDK3

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Validation Data Gallery

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  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12215-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12215-1-AP

  IP result of anti-PDK3 (IP:12215-1-AP, 4ug; Detection:12215-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human heart using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below

Product Information

12215-1-AP targets PDK3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDK3 fusion protein Ag2879
• Full Name: pyruvate dehydrogenase kinase, isozyme 3
• Calculated Molecular Weight: 406 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015948
• Gene Symbol: PDK3
• Gene ID (NCBI): 5165
• RRID: AB_2877836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDK3([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial) is also named as PDHK3 and belongs to the PDK/BCKDK protein kinase family. It inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 and 48 kDa. This protein can exsit as a dimer with the molecular weight of 90 kDa(PMID:11978179).

Publications

Species	Application	Title
human	WB	Cancer Res; IDH1 Mutation Induces Reprogramming of Pyruvate Metabolism.; Authors - Jose L Izquierdo-Garcia
human	IHC	Front Oncol; Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma; Authors - Yu-Hsuan Kuo
human	WB,IHC	Mol Cell Biochem; Lycorine hydrochloride interferes with energy metabolism to inhibit chemoresistant glioblastoma multiforme cell growth through suppressing PDK3; Authors - Qingsheng Dong
mouse	WB,IHC	Environ Pollut; Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse; Authors - Yan-Zhu Chen