PDILT Polyclonal antibody

PDILT Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26305-1-AP

Synonyms

PDIA7, PDILT

---

Validation Data Gallery

• 

  WB analysis using 26305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26305-1-AP (PDILT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours; mouse brain tissue, mouse heart tissue and mouse lung tissue are negative control.

• 

  WB analysis of mouse testis using 26305-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26305-1-AP (PDILT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 26305-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26305-1-AP (PDILT Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 26305-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26305-1-AP (PDILT Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse testis using 26305-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 26305-1-AP (PDILT antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 26305-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 26305-1-AP (PDILT antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26305-1-AP targets PDILT in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDILT fusion protein Ag23675
• Full Name: protein disulfide isomerase-like, testis expressed
• Observed Molecular Weight: 67 kDa, 75 kDa
• GenBank Accession Number: BC042607
• Gene Symbol: PDILT
• Gene ID (NCBI): 204474
• RRID: AB_2880472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDILT, a divergent testis-specific protein disulfide isomerase with a non-classical SXXC motif that engages in disulfide-dependent interactions in the endoplasmic reticulum (PMID: 15475357). PDILT interacts with CALR3 in the testicular germ cells and plays an indispensable role in the disulfide formation in the ADAM3. The PDILT knockout mice were male infertile, consistent with the previous observations that spermatozoa lacking ADAM3 cannot migrate through the UTJ and cannot bind to the ZP (PMID: 22357757). It can be detected as 67 kDa and 75kDa due to the glycosylation.