Filter: AllWBIHCIF

WB analysis using 66669-1-Ig (same clone as 66669-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66669-1-Ig (PDIA6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 66669-1-Ig (PDIA6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

PDIA6 Monoclonal antibody, PBS Only

PDIA6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66669-1-PBS

Clone No.1G2D4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

66669-1-PBS targets PDIA6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	PDIA6 fusion protein Ag27608 Predict reactive species
Full Name	protein disulfide isomerase family A, member 6
Calculated Molecular Weight	48 kDa, 54 kDa
Observed Molecular Weight	54 kDa
GenBank Accession Number	BC001312
Gene Symbol	PDIA6
Gene ID (NCBI)	10130
RRID	AB_2882023
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q15084
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

PDIA6 (Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze the formation, reduction, and isomerization of disulfide bonds in proteins and is thought to play a role in the folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.