PDIR Polyclonal antibody

• Featured Product
• KD/KO Validated

PDIR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15545-1-AP

Synonyms

PDIA5, PDIR, Protein disulfide isomerase A5

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Validation Data Gallery

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  WB analysis of mouse liver using 15545-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15545-1-AP (PDIR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15545-1-AP

  IP result of anti-PDIR (IP:15545-1-AP, 4ug; Detection:15545-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.

• 

  IHC staining of human placenta using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human placenta tissue, human liver tissue, human ovary tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

15545-1-AP targets PDIR in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDIR fusion protein Ag7901
• Full Name: protein disulfide isomerase family A, member 5
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC001625
• Gene Symbol: PDIR
• Gene ID (NCBI): 10954
• RRID: AB_2160995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Quantitative Proteomics and Differential Protein Abundance Analysis after the Depletion of PEX3 from Human Cells Identifies Additional Aspects of Protein Targeting to the ER.; Authors - Richard Zimmermann
human	WB,IHC	Oncotarget; Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.; Authors - Soma Samanta
human	WB	Viruses; PDIA4 Is a Host Factor Important for Lymphocytic Choriomeningitis Virus Infection; Authors - Mengwei Xu ; KD Validated
human	WB	FEBS J; Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.; Authors - Stefan Schorr
mouse	WB	JCI Insight; Protein disulfide isomerase is essential for spermatogenesis in mice; Authors - Yaqiong Zhang