PDHX Polyclonal antibody

• Featured Product
• KD/KO Validated

PDHX Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 10951-1-AP

Synonyms

PDX1, pyruvate dehydrogenase complex, component X, Lipoyl-containing pyruvate dehydrogenase complex component X, E3-binding protein, E3 binding protein

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Validation Data Gallery

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  WB analysis using 10951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 10951-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10951-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10951-1-AP

  IP result of anti-PDHX (IP:10951-1-AP, 4ug; Detection:10951-1-AP 1:5000) with HEK-293 cells lysate 2000ug.

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  IHC staining of human colon cancer using 10951-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10951-1-AP (PDHX antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HEK-293 cells, SKOV-3 cells, HepG2 cells, NIH/3T3 cells, mouse kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10951-1-AP targets PDHX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDHX fusion protein Ag1389
• Full Name: pyruvate dehydrogenase complex, component X
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC010389
• Gene Symbol: PDHX
• Gene ID (NCBI): 8050
• RRID: AB_2163102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDHX (Pyruvate dehydrogenase protein X component), an E3-binding protein in the PDC, is acetylated by the p300 at Lys 488, impeding the interaction between PDHX and dihydrolipoyl transacetylase (E2), thereby disrupting PDC assembly to inhibit its activation (PMID: 30012170). PDHX was characterized as a metabolically essential gene for esophageal squamous cell carcinoma (ESCC), and knockdown of PDHX inhibited the proliferation of cancer stem cells (CSCs) and in vivo tumor growth (PMID: 33964039). Western blot analysis detected PDHX at an apparent molecular mass of 54 kDa.

Publications

Species	Application	Title
	IF	J Hazard Mater; Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1; Authors - Hao Chen
human	WB,IHC,IF	Cancer Sci; Identification of PDHX as a metabolic target for esophageal squamous cell carcinoma.; Authors - Jun Inoue ; KD Validated
mouse	WB	Cell Calcium; The Mitochondrial Ca 2+ import complex is altered in ADPKD; Authors - Murali K Yanda
	WB	Front Microbiol; Singapore Grouper Iridovirus Induces Glucose Metabolism in Infected Cells by Activation of Mammalian Target of Rapamycin Signaling.; Authors - Xixi Guo ; KD Validated
rat	WB	Int J Biol Sci; Hypericin maintians PDX1 expression via the Erk pathway and protects islet β-cells against glucotoxicity and lipotoxicity.; Authors - Chen Liang
mouse	WB	PLoS One; Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.; Authors - Sol Eri Maria EM