PDHB Monoclonal antibody

PDHB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7C10

Cat no : 68238-1-Ig

Synonyms

PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1, 2A7C10

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Validation Data Gallery

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  WB analysis using 68238-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68238-1-Ig (PDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 68238-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 68238-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 68238-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 68238-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 68238-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDHB antibody (68238-1-Ig, Clone: 2A7C10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, mouse heart tissue
• Positive IHC detected in: mouse kidney tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68238-1-Ig targets PDHB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDHB fusion protein Ag6857
• Full Name: pyruvate dehydrogenase (lipoamide) beta
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000439
• Gene Symbol: PDHB
• Gene ID (NCBI): 5162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).