PDHB Polyclonal antibody

• Featured Product
• KD/KO Validated

PDHB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 14744-1-AP

Synonyms

PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1

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Validation Data Gallery

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  WB analysis using 14744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14744-1-AP (PDHB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14744-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14744-1-AP (PDHB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14744-1-AP

  IP result of anti-PDHB (IP:14744-1-AP, 4ug; Detection:14744-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 14744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14744-1-AP (PDHB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 14744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14744-1-AP (PDHB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14744-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PDHB antibody (14744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, human heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14744-1-AP targets PDHB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDHB fusion protein Ag6442
• Full Name: pyruvate dehydrogenase (lipoamide) beta
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000439
• Gene Symbol: PDHB
• Gene ID (NCBI): 5162
• RRID: AB_2162941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDHB(Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named as PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors -
human	WB	Mol Cell; Phosphorylation of PDHA by AMPK Drives TCA Cycle to Promote Cancer Metastasis.; Authors - Zhen Cai
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	Circ Res; (Pro)renin Receptor Inhibition Reprograms Hepatic Lipid Metabolism and Protects Mice from Diet-Induced Obesity and Hepatosteatosis.; Authors - Liwei Ren
mouse	WB	J Allergy Clin Immunol; Pyruvate dehydrogenase has a major role in mast cell function, and its activity is regulated by mitochondrial microphthalmia transcription factor.; Authors - Israa Sharkia